This 3-day course is a prime theoretical/hands-on training event on preparation of biological matrices including biofluids and tissues. Take the opportunity to learn from the experts and see what are the differences between the methods, what each of them can offer, learn that microextraction is more than just a few “simple” procedures. Understand the theoretical principles behind each technique, and learn how these principles can help you produce better results in your laboratory. There is no better or worst technique, you just need to understand what fits better your need. The course will cover aspects of exhaustive and microextraction procedures so you can understand challenges and avoid pitfalls when developing and applying different methods in your laboratory. What is important when doing targeted quantitative analysis and where to start if untargeted metabolite analysis is your goal. How to choose the best calibration for your application and how to validate your protocol. Course lectures will be followed by hands-on experiments covering solid phase extraction (SPE), solid-liquid extraction (SLE), liquid-liquid extraction (LLE), electromembrane extraction (EME) and several modes of liquid-phase microextraction (LPME). A complete method development strategy will be illustrated by real-life examples and coupling to gas chromatography (GC) and liquid chromatography (LC) will be introduced in detail. At every stage, the sample type and characteristics will be taken into consideration next to the green and cost-related aspects of sample preparation methods. You will also see the demonstrations of new strategies how to combine sample prep and fast instrumental analysis. Advanced experiments can also be arranged for more experienced users.
Why do you need to attend this course?
Sample preparation is the most critical and error prone part of an analytical method and any errors made cannot be corrected by using even the most advanced analytical instrumentation systems. During this course, top academic experts will give you a sound understanding of a comprehensive set of different extraction methods and teach you how to face challenges and avoid pitfalls when developing and applying them in your laboratory. This course will show you how to choose the “right” and “green” methodology as well as reduce laboratory costs and waste. You will have valuable opportunities to gain new skills and knowledge, improve existing ones as well as stay ahead of the competition. If you have reasonable gas and liquid chromatography experience, but want further hands-on training in various extraction approaches and keep up-to-date with the latest developments in the area of sample preparation, then this course is for you.
Who should attend?
The course is intended for both new and advanced industrial and testing lab analysts, PhD and MSc students, post-docs, lab managers and supervisors, academic based lab technicians and R&D staff working in bioanalytical, cosmetics, pharmaceutical, biotech or other analytical fields.
The aims of this course are:
-To build upon and extend the theoretical and experimental concepts on sample preparation including microextraction strategies
-To develop the competence and confidence of participants in various extraction methods
-To highlight modern advances in sample preparation within analytical chemistry
-To identify appropriate extraction technique for particular applications
After completing this seminar participants should be able to:
-Discuss and review in a comprehensive and critical way the methods available for converting liquid, solid and gaseous sample into a form suitable for the measurement step
-Identify the most suitable extraction method based on the type and characteristics of the sample
-Discuss the theoretical principles governing sample preparation/extraction
-Plan method development and interpret the effect of experimental parameters
-Identify measurement errors during the sample preparation analytical step
-Validate analytical methods and apply them to the analysis of real samples
-Interpret data and present the conclusions drawn in written and oral form
-Explain to non-specialists how given extraction approach can be expected to increase productivity and sensitivity, reduce laboratory costs and provide valuable information in different areas of chemistry and related disciplines.
-Identify green sample preparation methods